Aggrecan Polyclonal antibody

Aggrecan Polyclonal Antibody for IHC, FC (Intra), ELISA
Cat No. 13880-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (4)

应用

IHC, FC (Intra), ELISA and More (1)

ACAN, Cartilage-specific proteoglycan core protein, Aggrecan core protein 2, Aggrecan 1, AGCAN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman lung tissue, human cartilage tissue, human lung cancer tissue, mouse lung tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inATDC-5 cells
Planning an IHC experiment? We recommend our IHCeasy ACAN Ready-To-Use IHC Kit. ACAN primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13880-1-AP targets Aggrecan in IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, FC (Intra), ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, rabbit, canine, zebrafish
免疫原 Aggrecan fusion protein Ag4985 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aggrecan
别名 ACAN, Cartilage-specific proteoglycan core protein, Aggrecan core protein 2, Aggrecan 1, AGCAN
计算分子量 250 kDa
GenBank蛋白编号BC036445
基因名称 ACAN
Gene ID (NCBI) 176
RRIDAB_2722780
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16112
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

实验方案

Product Specific Protocols
IHC protocol for Aggrecan antibody 13880-1-APDownload protocol
FC protocol for Aggrecan antibody 13880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,IF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
human,mouseIF

Cell Stem Cell

A human organoid drug screen identifies α2-adrenergic receptor signaling as a therapeutic target for cartilage regeneration

Authors - Xiaocui Wei
human

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
human

J Extracell Vesicles

miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration.

Authors - Hongxing Hu
ratIF

Biomaterials

Injectable pathological microenvironment-responsive anti-inflammatory hydrogels for ameliorating intervertebral disc degeneration

Authors - Lei Liu
mouseIHC,IF

Bone Res

Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome

Authors - Yanqiu Wang
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