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验证数据展示

  • WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66596-1-Ig (same clone as 66596-1-PBS)

    IHC staining of human breast cancer using 66596-1-Ig (same clone as 66596-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66596-1-Ig (ACAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    WB result of ACAP1 antibody (66596-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ACAP1 Monoclonal antibody, PBS Only

ACAP1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66596-1-PBS

产品说明书

CloneNo. 1B4C9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66596-1-PBS targets ACAP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 ACAP1 fusion protein Ag14254 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
别名 Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
计算分子量 740 aa, 82 kDa
观测分子量 81 kDa
GenBank蛋白编号BC018543
基因名称 ACAP1
Gene ID (NCBI) 9744
RRIDAB_2881956
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15027
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.