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Species Reactivity
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验证数据展示

  • WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66596-1-Ig (ACAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66596-1-Ig (same clone as 66596-1-PBS)

    IHC staining of human breast cancer using 66596-1-Ig (same clone as 66596-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66596-1-Ig (ACAP1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    WB analysis of Jurkat using 66596-1-Ig (same clone as 66596-1-PBS)

    WB result of ACAP1 antibody (66596-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells. This data was developed using the same antibody clone with 66596-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ACAP1 Monoclonal antibody, PBS Only

ACAP1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66596-1-PBS
CloneNo. 1B4C9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66596-1-PBS targets ACAP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ACAP1 fusion protein Ag14254 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
RRIDAB_2881956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15027
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.