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  • Featured Product
  • KD/KO Validated

ACAP1 Recombinant antibody, PBS Only

ACAP1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230370E1

Cat no : 82967-1-PBS

Print datasheet

Synonyms

Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1

验证数据展示

  • WB analysis of Jurkat using 82967-1-RR (same clone as 82967-1-PBS)

    WB analysis of Jurkat using 82967-1-RR (same clone as 82967-1-PBS)

    WB result of ACAP1 antibody (82967-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAP1 transfected Jurkat cells. This data was developed using the same antibody clone with 82967-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 82967-1-RR (same clone as 82967-1-PBS)

    WB analysis of Jurkat using 82967-1-RR (same clone as 82967-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 82967-1-RR (ACAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82967-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 82967-1-RR (same clone as 82967-1-PBS)

    WB analysis of K-562 using 82967-1-RR (same clone as 82967-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 82967-1-RR (ACAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82967-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82967-1-RR

    Affinity and Kinetic Characterization of 82967-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82967-1-RR against Human ACAP1 were performed. The affinity constant is 0.42 nM.

产品信息

82967-1-PBS targets ACAP1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ACAP1 fusion protein Ag34309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms Cnt b1, CENTB1, Centaurin-beta-1, Centaurin beta 1, Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight 82-85 kDa
GenBank Accession NumberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15027
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.