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  • Featured Product
  • KD/KO Validated

ACAT1 Polyclonal antibody, PBS Only

ACAT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16215-1-PBS

Print datasheet

Synonyms

MAT, EC:2.3.1.9, Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, Acetoacetyl CoA thiolase

验证数据展示

  • WB analysis of MCF-7 using 16215-1-AP (same clone as 16215-1-PBS)

    WB analysis of MCF-7 using 16215-1-AP (same clone as 16215-1-PBS)

    WB result of ACAT1 antibody (16215-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • WB analysis using 16215-1-AP (same clone as 16215-1-PBS)

    WB analysis using 16215-1-AP (same clone as 16215-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16215-1-AP (ACAT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

    IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACAT1 antibody included
  • FC experiment of HeLa using 16215-1-AP (same clone as 16215-1-PBS)

    FC experiment of HeLa using 16215-1-AP (same clone as 16215-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACAT1 (16215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 16215-1-AP (same clone as 16215-1-PBS)

    IP experiment of mouse heart using 16215-1-AP (same clone as 16215-1-PBS)

    IP result of anti-ACAT1 (IP:16215-1-AP, 4ug; Detection:16215-1-AP 1:500) with mouse heart tissue lysate 4400ug. This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

    IHC staining of human liver using 16215-1-AP (same clone as 16215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16215-1-AP (ACAT1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACAT1 antibody included
  • IHC staining of human colon using 16215-1-AP (same clone as 16215-1-PBS)

    IHC staining of human colon using 16215-1-AP (same clone as 16215-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon slide using 16215-1-AP (ACAT1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACAT1 antibody included
  • IF Staining of HeLa using 16215-1-AP (same clone as 16215-1-PBS)

    IF Staining of HeLa using 16215-1-AP (same clone as 16215-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ACAT1 antibody (16215-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16215-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ACAT1 Ready-To-Use IHC Kit. ACAT1 primary antibody included.

产品信息

16215-1-PBS targets ACAT1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACAT1 fusion protein Ag9215 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acetyl-Coenzyme A acetyltransferase 1
Synonyms MAT, EC:2.3.1.9, Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, Acetoacetyl CoA thiolase
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 38-45 kDa
GenBank Accession NumberBC010942
Gene Symbol ACAT1
Gene ID (NCBI) 38
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24752
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACAT1 (acetyl-CoA acetyltransferase), is a mitochondrial enzyme catalyzing the reversible formation or breakdown of acetoacetyl-CoA and plays important roles in ketogenesis and ketolysis. Deficiency of ACAT1 causes an inherited metabolic disorder. Recently ACAT1 has been reported to get involved in cancer progression.