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验证数据展示

  • WB analysis using 68005-1-Ig

    WB analysis using 68005-1-Ig

    Various cell lysates were subjected to SDS PAGE followed by western blot with 68005-1-Ig (ACAT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human placenta using 68005-1-Ig

    IHC staining of human placenta using 68005-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68005-1-Ig (ACAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 68005-1-Ig

    IF Staining of HepG2 using 68005-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (68005-1-Ig, Clone: 1F5F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of HepG2 using 68005-1-Ig

    WB analysis of HepG2 using 68005-1-Ig

    WB result of ACAT2 antibody (68005-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

ACAT2 Monoclonal antibody

ACAT2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68005-1-Ig

产品说明书

CloneNo. 1F5F11
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit

应用

WB, IHC, IF/ICC, ELISA

别名

ACAT2, ACTL

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HCT 116 cells, rat brain tissue, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue, rabbit brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68005-1-Ig targets ACAT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat, rabbit
文献引用反应性mouse
免疫原 ACAT2 fusion protein Ag6596 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 acetyl-Coenzyme A acetyltransferase 2
别名 ACAT2, ACTL
计算分子量 41 kDa
观测分子量42 kDa
GenBank蛋白编号BC000408
基因名称 ACAT2
Gene ID (NCBI) 39
RRIDAB_2918752
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BWD1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACAT2 antibody 68005-1-IgDownload protocol
IHC protocol for ACAT2 antibody 68005-1-IgDownload protocol
IF protocol for ACAT2 antibody 68005-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Phytomedicine

Investigating the potential mechanism and therapeutic effects of SLXG for cholesterol gallstone treatment

Authors - Yang Wang
View Article