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ACBD3 Polyclonal antibody

ACBD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14096-1-AP

Print datasheet

Synonyms

Golgi resident protein GCP60, Golgi phosphoprotein 1, Golgi complex-associated protein 1, GOCAP1, GCP60

验证数据展示

  • IHC staining of mouse testis using 14096-1-AP

    IHC staining of mouse testis using 14096-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14096-1-AP (ACBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14096-1-AP

    WB analysis using 14096-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14096-1-AP (ACBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14096-1-AP

    IF Staining of HeLa using 14096-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14096-1-AP (ACBD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, mouse ovary tissue, mouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14096-1-AP targets ACBD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ACBD3 fusion protein Ag5228 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-Coenzyme A binding domain containing 3
Synonyms Golgi resident protein GCP60, Golgi phosphoprotein 1, Golgi complex-associated protein 1, GOCAP1, GCP60
Calculated Molecular Weight 528 aa, 61 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC060792
Gene Symbol ACBD3
Gene ID (NCBI) 64746
RRIDAB_2220062
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3P7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACBD3 antibody 14096-1-APDownload protocol
IHC protocol for ACBD3 antibody 14096-1-APDownload protocol
IF protocol for ACBD3 antibody 14096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Biol Int

Golgi protein ACBD3 downregulation sensitizes cells to ferroptosis

Authors - Ying Qian
  • KD Validated
View Article