验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse skeletal muscle tissue, L02 cells, MCF-7 cells |
| Positive IHC detected in | human ovary cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
21080-1-AP targets ACBD5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | ACBD5 fusion protein Ag15287 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acyl-Coenzyme A binding domain containing 5 |
| 别名 | KIAA1996, Acyl-CoA-binding domain-containing protein 5 |
| 计算分子量 | 534 aa, 60 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC030555 |
| 基因名称 | ACBD5 |
| Gene ID (NCBI) | 91452 |
| RRID | AB_2878808 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T8D3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACBD5, also known as RDLKD. It is expected to be located in the peroxisome membrane, which is ubiquitinated in brain and kidney tissue. This gene encodes a member of the acyl-Coenzyme A binding protein family, known to function in the transport and distribution of long chain acyl-Coenzyme A in cells. This gene may play a role in the differentiation of megakaryocytes and formation of platelets. A related protein in yeast is involved in autophagy of peroxisomes. A mutation in this gene has been associated with autosomal dominant thrombocytopenia. The molecular weight of ACBD5 is 60 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACBD5 antibody 21080-1-AP | Download protocol |
| IHC protocol for ACBD5 antibody 21080-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways. | ||
Mol Cell Biochem Differential distribution of peroxisomal proteins points to specific roles of peroxisomes in the murine retina. | ||
