ACC1 Recombinant antibody
ACC1 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
2I13
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, C6 cells |
Positive IP detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81656-1-RR targets ACC1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | ACC1 fusion protein Ag16452 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | acetyl-Coenzyme A carboxylase alpha |
Synonyms | ACACA, ACC-alpha, ACCA, ACC, ACAC |
Calculated Molecular Weight | 2383 aa, 275 kDa |
Observed Molecular Weight | 250 kDa |
GenBank Accession Number | BC137287 |
Gene Symbol | Acetyl-CoA Carboxylase 1 |
Gene ID (NCBI) | 31 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13085 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for ACC1 antibody 81656-1-RR | Download protocol |
IP protocol for ACC1 antibody 81656-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |