ACC1 Recombinant antibody

ACC1 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2I13

Cat no : 81656-1-RR

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Synonyms

ACACA, ACC-alpha, ACCA, ACC, ACAC



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, C6 cells
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81656-1-RR targets ACC1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACC1 fusion protein Ag16452 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acetyl-Coenzyme A carboxylase alpha
Synonyms ACACA, ACC-alpha, ACCA, ACC, ACAC
Calculated Molecular Weight 2383 aa, 275 kDa
Observed Molecular Weight 250 kDa
GenBank Accession NumberBC137287
Gene Symbol Acetyl-CoA Carboxylase 1
Gene ID (NCBI) 31
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13085
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

实验方案

Product Specific Protocols
WB protocol for ACC1 antibody 81656-1-RRDownload protocol
IP protocol for ACC1 antibody 81656-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols