• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ACC1 Monoclonal antibody, PBS Only

ACC1 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A11G10

Cat No : 67373-1-PBS

Print datasheet

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1

验证数据展示

  • WB analysis using 67373-1-Ig (same clone as 67373-1-PBS)

    WB analysis using 67373-1-Ig (same clone as 67373-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67373-1-Ig (ACC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67373-1-Ig (same clone as 67373-1-PBS)

    WB analysis of HeLa using 67373-1-Ig (same clone as 67373-1-PBS)

    WB result of ACC1 antibody (67373-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACC1 transfected HeLa cells. This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67373-1-Ig (same clone as 67373-1-PBS)

    IF Staining of HeLa using 67373-1-Ig (same clone as 67373-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ACC1 antibody (67373-1-Ig, Clone: 1A11G10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67373-1-Ig (same clone as 67373-1-PBS)

    IHC staining of human breast cancer using 67373-1-Ig (same clone as 67373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67373-1-Ig (same clone as 67373-1-PBS)

    IHC staining of human breast cancer using 67373-1-Ig (same clone as 67373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67373-1-Ig (ACC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67373-1-PBS in a different storage buffer formulation.

产品信息

67373-1-PBS targets ACC1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ACC1 fusion protein Ag17503 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name acetyl-Coenzyme A carboxylase alpha
Synonyms ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1
Calculated Molecular Weight 2383 aa, 275 kDa
Observed Molecular Weight 250-270 kDa
GenBank Accession NumberBC137287
Gene Symbol ACACA
Gene ID (NCBI) 31
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13085
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.