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PHACS Polyclonal antibody

PHACS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11491-1-AP

Print datasheet

Synonyms

ACC synthase like protein 1, ACCS, ACS, PHACS

验证数据展示

  • WB analysis of MDA-MB-453s using 11491-1-AP

    WB analysis of MDA-MB-453s using 11491-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11491-1-AP

    IHC staining of human colon cancer using 11491-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11491-1-AP (PHACS antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inMDA-MB-453s cells, HeLa cells, mouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11491-1-AP targets PHACS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PHACS fusion protein Ag2056 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 1-aminocyclopropane-1-carboxylate synthase homolog (Arabidopsis)(non-functional)
Synonyms ACC synthase like protein 1, ACCS, ACS, PHACS
Calculated Molecular Weight 501 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC020197
Gene Symbol PHACS
Gene ID (NCBI) 84680
RRIDAB_2222681
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACCS(1-aminocyclopropane-1-carboxylate synthase-like protein 1) is also named as PHACS and belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. The deduced 501-amino acid protein has a calculated molecular mass of 58 kD. It contains overlapping aminotransferase I and beta-eliminating lyase domains, and it shares structural similarity with aspartate aminotransferase, tyrosine aminotransferase, and enzymes that catalyze beta-elimination reactions on amino acids.

实验方案

Product Specific Protocols
WB protocol for PHACS antibody 11491-1-APDownload protocol
IHC protocol for PHACS antibody 11491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols