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验证数据展示

  • WB analysis using 30522-1-AP

    WB analysis using 30522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30522-1-AP (ACIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 30522-1-AP

    IF Staining of mouse cerebellum using 30522-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ACIN1-L specific antibody (30522-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ACIN1-L specific Polyclonal antibody

ACIN1-L specific Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30522-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IF-P, ELISA

别名

ACIN1, ACINUS, ACN, DKFZp667N107, fSAP152, KIAA0670

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30522-1-AP targets ACIN1-L specific in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 Human, mouse
免疫原 ACIN1-L specific fusion protein Ag30506 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apoptotic chromatin condensation inducer 1
别名 ACIN1, ACINUS, ACN, DKFZp667N107, fSAP152, KIAA0670
计算分子量 1341 aa, 152 kDa
观测分子量240 kDa
GenBank蛋白编号BC140805
基因名称 ACIN1
Gene ID (NCBI) 22985
RRIDAB_3669730
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKV3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. ACIN1 is a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. There are three isoforms of human acinus (the L, S and S' forms) shows at about 80-85 kDa and 220-250 kDa by Western Blot, which were probably generated by alternative splicing (PMID: 10490026). 30522-1-AP specificly targets at the ACIN1-L, while another antibody 23937-1-AP can detect all three isoforms of ACIN1.

实验方案

Product Specific Protocols
WB protocol for ACIN1-L specific antibody 30522-1-APDownload protocol
IF protocol for ACIN1-L specific antibody 30522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols