• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of A549 using 14304-1-AP (same clone as 14304-1-PBS)

    WB analysis of A549 using 14304-1-AP (same clone as 14304-1-PBS)

    WB result of ACK1 antibody (14304-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACK1 transfected A549 cells. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 14304-1-AP (same clone as 14304-1-PBS)

    WB analysis of SH-SY5Y using 14304-1-AP (same clone as 14304-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNK2/ACK1 antibody included
  • IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNK2/ACK1 antibody included
  • Featured Product
  • KD/KO验证

ACK1 Polyclonal antibody, PBS Only

ACK1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14304-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TNK2/ACK1 Ready-To-Use IHC Kit. TNK2/ACK1 primary antibody included.

产品信息

14304-1-PBS targets ACK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACK1 fusion protein Ag5419 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosine kinase, non-receptor, 2
别名
计算分子量 115 kDa
观测分子量 70 kDa
GenBank蛋白编号BC028164
基因名称 TNK2
Gene ID (NCBI) 10188
RRIDAB_11041713
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07912
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNK2, also named ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, and cell proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.