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  • Featured Product
  • KD/KO Validated

ACK1 Polyclonal antibody, PBS Only

ACK1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14304-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of A549 using 14304-1-AP (same clone as 14304-1-PBS)

    WB analysis of A549 using 14304-1-AP (same clone as 14304-1-PBS)

    WB result of ACK1 antibody (14304-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACK1 transfected A549 cells. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 14304-1-AP (same clone as 14304-1-PBS)

    WB analysis of SH-SY5Y using 14304-1-AP (same clone as 14304-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNK2/ACK1 antibody included
  • IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    IHC staining of human breast cancer using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNK2/ACK1 antibody included
Planning an IHC experiment? We recommend our IHCeasy TNK2/ACK1 Ready-To-Use IHC Kit. TNK2/ACK1 primary antibody included.

产品信息

14304-1-PBS targets ACK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACK1 fusion protein Ag5419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tyrosine kinase, non-receptor, 2
Synonyms
Calculated Molecular Weight 115 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC028164
Gene Symbol TNK2
Gene ID (NCBI) 10188
RRIDAB_11041713
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07912
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNK2, also named ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, and cell proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.