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ACO2 Monoclonal antibody, PBS Only

ACO2 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F1G4

Cat no : 67509-1-PBS

Print datasheet

Synonyms

aconitase 2, Citrate hydro-lyase, Aconitase2, Aconitase, ACO 2

验证数据展示

  • WB analysis using 67509-1-Ig (same clone as 67509-1-PBS)

    WB analysis using 67509-1-Ig (same clone as 67509-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67509-1-Ig (same clone as 67509-1-PBS)

    IHC staining of human liver cancer using 67509-1-Ig (same clone as 67509-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACO2 antibody included
  • IHC staining of human liver cancer using 67509-1-Ig (same clone as 67509-1-PBS)

    IHC staining of human liver cancer using 67509-1-Ig (same clone as 67509-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67509-1-Ig (ACO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACO2 antibody included
  • FC experiment of HeLa using 67509-1-Ig (same clone as 67509-1-PBS)

    FC experiment of HeLa using 67509-1-Ig (same clone as 67509-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ACO2 (67509-1-Ig, Clone:1F1G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

  • WB analysis using 67509-1-Ig (same clone as 67509-1-PBS)

    WB analysis using 67509-1-Ig (same clone as 67509-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67509-1-Ig (same clone as 67509-1-PBS)

    WB analysis of HEK-293 using 67509-1-Ig (same clone as 67509-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67509-1-Ig (ACO2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67509-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ACO2 Ready-To-Use IHC Kit. ACO2 primary antibody included.

产品信息

67509-1-PBS targets ACO2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ACO2 fusion protein Ag17784 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aconitase 2, mitochondrial
Synonyms aconitase 2, Citrate hydro-lyase, Aconitase2, Aconitase, ACO 2
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC014092
Gene Symbol ACO2
Gene ID (NCBI) 50
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99798
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).