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验证数据展示

  • IF Staining of A431 using 10776-1-AP

    IF Staining of A431 using 10776-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 10776-1-AP (ACOT11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human prostate cancer using 10776-1-AP

    IHC staining of human prostate cancer using 10776-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10776-1-AP (ACOT11 antibody) at dilution of 1:100 (under 10x lens).

  • WB analysis using 10776-1-AP

    WB analysis using 10776-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10776-1-AP (ACOT11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ACOT11 Polyclonal antibody

ACOT11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10776-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

BFIT, Adipose-associated thioesterase, Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, Acyl-CoA thioester hydrolase 11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10776-1-AP targets ACOT11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ACOT11 fusion protein Ag1176 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA thioesterase 11
别名 BFIT, Adipose-associated thioesterase, Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, Acyl-CoA thioester hydrolase 11
计算分子量68.4 kDa
观测分子量68-70 kDa
GenBank蛋白编号BC001517
基因名称 ACOT11
Gene ID (NCBI) 26027
RRIDAB_2257798
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WXI4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOT11(Acyl-coenzyme A thioesterase 11) is also named as BFIT, KIAA0707, THEA.BFIT is mapped to syntenic regions of chromosomes 1 (human) and 4 (mouse) associated with body fatness and diet-induced obesity, potentially linking a deficit of BFIT activity with exacerbation of these traits and supports the transition of this tissue towards increased metabolic activity, probably through alteration of intracellular fatty acyl-CoA concentration(PMID:11696000).

实验方案

Product Specific Protocols
WB protocol for ACOT11 antibody 10776-1-APDownload protocol
IHC protocol for ACOT11 antibody 10776-1-APDownload protocol
IF protocol for ACOT11 antibody 10776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Thorac Dis

Establishment and validation of a prognostic model for idiopathic pulmonary fibrosis based on mitochondrial-related genes

Authors - Xuewen Wang
View Article