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验证数据展示

  • WB analysis using 17871-1-AP

    WB analysis using 17871-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17871-1-AP (ACOT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 17871-1-AP

    IHC staining of human colon cancer using 17871-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17871-1-AP (ACOT12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 17871-1-AP

    IF Staining of U2OS using 17871-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ACOT12 antibody (17871-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ACOT12 Polyclonal antibody

ACOT12 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17871-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Acetyl-coenzyme A thioesterase, ACOT 12, Acyl-CoA thioester hydrolase 12, Acyl-CoA thioesterase 12, Acyl-coenzyme A thioesterase 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HuH-7 cells, rat liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17871-1-AP targets ACOT12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 ACOT12 fusion protein Ag12355 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA thioesterase 12
别名 Acetyl-coenzyme A thioesterase, ACOT 12, Acyl-CoA thioester hydrolase 12, Acyl-CoA thioesterase 12, Acyl-coenzyme A thioesterase 12
计算分子量 555 aa, 62 kDa
观测分子量 70 kDa
GenBank蛋白编号BC075011
基因名称 ACOT12
Gene ID (NCBI) 134526
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WYK0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOT12 is an acyl-coenzyme A thioesterase that hydrolyzes acyl-coenzyme A (acyl-CoA) to free fatty acids and coenzyme A, thereby regulating intracellular levels of acyl-CoA and free fatty acids. It plays a key role in lipid metabolism and is mainly expressed in the kidney, liver, and intestine. ACOT12 plays an important role in a variety of diseases, including renal fibrosis, nonalcoholic fatty liver disease (NAFLD), and hepatocellular carcinoma. In renal fibrosis, downregulation of ACOT12 expression leads to lipid accumulation and exacerbates the fibrotic process. Restoration of its expression ameliorates renal fibrosis, suggesting that ACOT12 is a potential therapeutic target.

实验方案

Product Specific Protocols
WB protocol for ACOT12 antibody 17871-1-APDownload protocol
IHC protocol for ACOT12 antibody 17871-1-APDownload protocol
IF protocol for ACOT12 antibody 17871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols