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Species Reactivity
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验证数据展示

  • WB analysis using 17871-1-AP

    WB analysis using 17871-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17871-1-AP (ACOT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ACOT12 Polyclonal antibody

ACOT12 Polyclonal Antibody for WB, ELISA
Cat No. 17871-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

CACH, Acyl-coenzyme A thioesterase 12, Acyl-CoA thioesterase 12, Acyl-CoA thioester hydrolase 12, ACOT 12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HuH-7 cells, rat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17871-1-AP targets ACOT12 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen ACOT12 fusion protein Ag12355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-CoA thioesterase 12
Synonyms CACH, Acyl-coenzyme A thioesterase 12, Acyl-CoA thioesterase 12, Acyl-CoA thioester hydrolase 12, ACOT 12
Calculated Molecular Weight 555 aa, 62 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC075011
Gene Symbol ACOT12
Gene ID (NCBI) 134526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOT12 is an acyl-coenzyme A thioesterase that hydrolyzes acyl-coenzyme A (acyl-CoA) to free fatty acids and coenzyme A, thereby regulating intracellular levels of acyl-CoA and free fatty acids. It plays a key role in lipid metabolism and is mainly expressed in the kidney, liver, and intestine. ACOT12 plays an important role in a variety of diseases, including renal fibrosis, nonalcoholic fatty liver disease (NAFLD), and hepatocellular carcinoma. In renal fibrosis, downregulation of ACOT12 expression leads to lipid accumulation and exacerbates the fibrotic process. Restoration of its expression ameliorates renal fibrosis, suggesting that ACOT12 is a potential therapeutic target.

实验方案

Product Specific Protocols
WB protocol for ACOT12 antibody 17871-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols