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ACOT12 Polyclonal antibody

ACOT12 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17871-1-AP

Print datasheet

Synonyms

ACOT12, acyl CoA thioesterase 12, Cach, CACH 1, CACH1, hCACH 1, STARD15, THEAL

验证数据展示

  • WB analysis of COLO 320 using 17871-1-AP

    WB analysis of COLO 320 using 17871-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17871-1-AP (ACOT12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17871-1-AP

    IHC staining of human liver cancer using 17871-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17871-1-AP (ACOT12 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 17871-1-AP

    IHC staining of human liver cancer using 17871-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17871-1-AP (ACOT12 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of COLO 320 using 17871-1-AP

    IF Staining of COLO 320 using 17871-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 17871-1-AP (ACOT12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOLO 320 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17871-1-AP is ACOT12 Fusion Protein expressed in E. coli.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACOT12 fusion protein Ag12355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-CoA thioesterase 12
Synonyms ACOT12, acyl CoA thioesterase 12, Cach, CACH 1, CACH1, hCACH 1, STARD15, THEAL
Calculated Molecular Weight 555 aa, 62 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC075011
Gene Symbol ACOT12
Gene ID (NCBI) 134526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.