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验证数据展示

  • WB analysis of L02 using 17316-1-AP

    WB analysis of L02 using 17316-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 17316-1-AP

    WB analysis using 17316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 17316-1-AP

    IHC staining of mouse kidney using 17316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 17316-1-AP

    IHC staining of mouse kidney using 17316-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 17316-1-AP

    IF Staining of A549 using 17316-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOT4 antibody (17316-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

ACOT4 Polyclonal antibody

ACOT4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17316-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ACOT4, acyl CoA thioesterase 4, Acyl coenzyme A thioesterase 4, PTE 2b, PTE Ib, PTE1B, PTE2B, PTEIB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17316-1-AP targets ACOT4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ACOT4 fusion protein Ag11286 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA thioesterase 4
别名 ACOT4, acyl CoA thioesterase 4, Acyl coenzyme A thioesterase 4, PTE 2b, PTE Ib, PTE1B, PTE2B, PTEIB
计算分子量 421 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC031799
基因名称 ACOT4
Gene ID (NCBI) 122970
RRIDAB_3085521
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N9L9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOT4, also known as PET2B and PTEIB, is an acyl-CoA thioesterases which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), regulating their respective intracellular levels. ACOT4 has the strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle(PMID: 16940157).

实验方案

Product Specific Protocols
WB protocol for ACOT4 antibody 17316-1-APDownload protocol
IHC protocol for ACOT4 antibody 17316-1-APDownload protocol
IF protocol for ACOT4 antibody 17316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols