ACOT4 Polyclonal antibody
ACOT4 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, L02 cells |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A549 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17316-1-AP targets ACOT4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF/ICC, IHC, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | ACOT4 fusion protein Ag11286 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | acyl-CoA thioesterase 4 |
Synonyms | ACOT4, acyl CoA thioesterase 4, Acyl coenzyme A thioesterase 4, PTE 2b, PTE Ib, PTE1B, PTE2B, PTEIB |
Calculated Molecular Weight | 421 aa, 46 kDa |
Observed Molecular Weight | 46 kDa |
GenBank Accession Number | BC031799 |
Gene Symbol | ACOT4 |
Gene ID (NCBI) | 122970 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8N9L9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ACOT4 antibody 17316-1-AP | Download protocol |
IHC protocol for ACOT4 antibody 17316-1-AP | Download protocol |
IF protocol for ACOT4 antibody 17316-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |