验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, mouse brain tissue, human brain tissue, Jurkat cells, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15972-1-AP targets ACOT7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, bovine |
| 免疫原 | ACOT7 fusion protein Ag8759 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acyl-CoA thioesterase 7 |
| 别名 | ACH1, ACOT7, ACT, acyl CoA thioesterase 7, BACH, Brain acyl CoA hydrolase, CTE II, CTE IIa, hBACH, LACH, LACH1 |
| 计算分子量 | 370 aa, 40 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC017365 |
| 基因名称 | ACOT7 |
| Gene ID (NCBI) | 11332 |
| RRID | AB_2221652 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00154 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:10578051). Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (PubMed:10578051). May play an important physiological function in brain (PubMed:10578051). ACOT7 has 7 isoforms between 27-40 kDa, we can detect a band at 37 kDa. ACOT7 is high homology with ACOT7L, this antibody may also detect ACOT7L.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACOT7 antibody 15972-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Convergent genomic signatures of flight loss in birds suggest a switch of main fuel. | ||
J Hepatocell Carcinoma Acyl-CoA Thioesterase 7 is Transcriptionally Activated by Krüppel-Like Factor 13 and Promotes the Progression of Hepatocellular Carcinoma. | ||
J Proteomics TMT-labeled quantitative malonylome analysis on the longissimus dorsi muscle of Laiwu pigs reveals the role of ACOT7 in fat deposition | ||
Acta Histochem ACOT7 positively regulated by CREB1 promotes the progression of cutaneous melanoma | ||
Int J Mol Sci Role of Acyl-CoA Thioesterase 7 in Regulating Fatty Acid Metabolism and Its Contribution to the Onset and Progression of Bovine Clinical Mastitis | ||
In Vitro Cell Dev Biol Anim ACOT7 protects epidermal stem cells against lipid peroxidation |
