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验证数据展示

  • WB analysis using 66532-1-Ig

    WB analysis using 66532-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66532-1-Ig (ACOT9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 66532-1-Ig

    IHC staining of human colon cancer using 66532-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66532-1-Ig (ACOT9 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ACOT9 antibody included
  • IHC staining of human colon cancer using 66532-1-Ig

    IHC staining of human colon cancer using 66532-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66532-1-Ig (ACOT9 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ACOT9 antibody included

ACOT9 Monoclonal antibody

ACOT9 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66532-1-Ig

产品说明书

CloneNo. 3F10G5
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Rat, mouse and More (1)

应用

WB, IHC, ELISA

别名

ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, RAW 264.7 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ACOT9 Ready-To-Use IHC Kit. ACOT9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66532-1-Ig targets ACOT9 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Rat, mouse
文献引用反应性human
免疫原 ACOT9 fusion protein Ag8884 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 acyl-CoA thioesterase 9
别名 ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48
计算分子量 24 kDa, 49 kDa
观测分子量 43-50 kDa
GenBank蛋白编号BC012573
基因名称 ACOT9
Gene ID (NCBI) 23597
RRIDAB_2881895
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y305
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.

实验方案

Product Specific Protocols
WB protocol for ACOT9 antibody 66532-1-IgDownload protocol
IHC protocol for ACOT9 antibody 66532-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma

Authors - Qifan Hu
View Article