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ACOX3 Recombinant antibody

ACOX3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241172G8

Cat no : 84027-4-RR

Print datasheet

Synonyms

EC:1.3.3.6, BRCOX, BRCACox, Branched-chain acyl-CoA oxidase, ACOX 3

验证数据展示

  • WB analysis using 84027-4-RR

    WB analysis using 84027-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84027-4-RR (ACOX3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84027-4-RR

    FC experiment of A431 using 84027-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX3 Recombinant antibody (84027-4-RR, Clone:241172G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84027-4-RR

    Affinity and Kinetic Characterization of 84027-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84027-4-RR against Human ACOX3 were performed. The affinity constant is 1.05 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HL-60 cells, U-251 cells, A549 cells，human placenta tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84027-4-RR targets ACOX3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ACOX3 fusion protein Ag10553 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acyl-Coenzyme A oxidase 3, pristanoyl
Synonyms EC:1.3.3.6, BRCOX, BRCACox, Branched-chain acyl-CoA oxidase, ACOX 3
Calculated Molecular Weight 624 aa, 70 kDa
Observed Molecular Weight70-78 kDa
GenBank Accession NumberBC017053
Gene Symbol ACOX3
Gene ID (NCBI) 8310
RRIDAB_3671594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15254
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acyl-Coenzyme A oxidase 3 also know as pristanoyl -CoA oxidase (ACOX3) is involved in the desaturation of 2-methyl branched fatty acids in peroxisomes.

实验方案

Product Specific Protocols
WB protocol for ACOX3 antibody 84027-4-RRDownload protocol
FC protocol for ACOX3 antibody 84027-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols