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ACP1 Polyclonal antibody

ACP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 22214-1-AP

Print datasheet

Synonyms

acid phosphatase 1, soluble, ACP1, Adipocyte acid phosphatase, HAAP, LMW PTP, LMW PTPase, Red cell acid phosphatase 1

验证数据展示

  • WB analysis of mouse brain using 22214-1-AP

    WB analysis of mouse brain using 22214-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22214-1-AP (ACP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22214-1-AP

    IHC staining of human breast cancer using 22214-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 22214-1-AP

    IHC staining of human breast cancer using 22214-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22214-1-AP (ACP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

22214-1-AP targets ACP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ACP1 fusion protein Ag17539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acid phosphatase 1, soluble
Synonyms acid phosphatase 1, soluble, ACP1, Adipocyte acid phosphatase, HAAP, LMW PTP, LMW PTPase, Red cell acid phosphatase 1
Calculated Molecular Weight 158 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC007422
Gene Symbol ACP1
Gene ID (NCBI) 52
RRIDAB_2879032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24666
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human red cell acid phosphatase (ACP1) is a polymorphic enzyme closely related to cytosolic low molecular weight acid phosphatases, a protein family broadly conserved among eukaryotes. It catalyses the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACP1 antibody 22214-1-APDownload protocol
IHC protocol for ACP1 antibody 22214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules

Authors - Tao Wang
View Article