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ACP2 Polyclonal antibody

ACP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15236-1-AP

Print datasheet

Synonyms

LAP, EC:3.1.3.2, ACP 2

验证数据展示

  • WB analysis of mouse kidney using 15236-1-AP

    WB analysis of mouse kidney using 15236-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15236-1-AP (ACP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15236-1-AP

    IF Staining of HepG2 using 15236-1-AP

    Immunofluorescent analysis of HepG2 cells using 15236-1-AP (ACP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse kidney tissue, HepG2 cells, HL-60 cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15236-1-AP targets ACP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACP2 fusion protein Ag7356 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acid phosphatase 2, lysosomal
Synonyms LAP, EC:3.1.3.2, ACP 2
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 63 kDa, 52 kDa
GenBank Accession NumberBC003160
Gene Symbol ACP2
Gene ID (NCBI) 53
RRIDAB_2221939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11117
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACP2 antibody 15236-1-APDownload protocol
IF protocol for ACP2 antibody 15236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols