验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, COLO 320 cells, MCF-7 cells, mouse lung tissue, mouse testis tissue |
| Positive IHC detected in | human prostate cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15840-1-AP targets ACPP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse, rat, chicken |
| 免疫原 | ACPP fusion protein Ag8592 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acid phosphatase, prostate |
| 别名 | acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase |
| 计算分子量 | 418 aa, 48 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC007460 |
| 基因名称 | ACPP |
| Gene ID (NCBI) | 55 |
| RRID | AB_2222099 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P15309 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase, thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACPP antibody 15840-1-AP | Download protocol |
| IHC protocol for ACPP antibody 15840-1-AP | Download protocol |
| IF protocol for ACPP antibody 15840-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biol Reprod Avian Prostatic Acid Phosphatase: Estrogen Regulation in the Oviduct and Epithelial Cell-Derived Ovarian Carcinomas. | ||
Am J Pathol PIK3CG Is a Potential Therapeutic Target in Androgen Receptor-Indifferent Metastatic Prostate Cancer. | ||
Eur J Pain Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice. | ||
Biochem Biophys Res Commun Dietary cholesterol affects expression of prostatic acid phosphatase in reproductive organs of male rats. |
