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ACSBG1 Polyclonal antibody

ACSBG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16077-1-AP

Print datasheet

Synonyms

ACSBG1, BG1, BGM, GR LACS, hBG1, hsBG, hsBGM, KIAA0631, Lipodisin, LPD

验证数据展示

  • WB analysis of mouse testis using 16077-1-AP

    WB analysis of mouse testis using 16077-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16077-1-AP (ACSBG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 16077-1-AP

    IHC staining of mouse testis using 16077-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16077-1-AP (ACSBG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 16077-1-AP

    IHC staining of mouse testis using 16077-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16077-1-AP (ACSBG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16077-1-AP targets ACSBG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ACSBG1 fusion protein Ag9043 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-CoA synthetase bubblegum family member 1
Synonyms ACSBG1, BG1, BGM, GR LACS, hBG1, hsBG, hsBGM, KIAA0631, Lipodisin, LPD
Calculated Molecular Weight 724 aa, 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC009289
Gene Symbol ACSBG1
Gene ID (NCBI) 23205
RRIDAB_2878214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GR2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACSBG1 antibody 16077-1-APDownload protocol
IHC protocol for ACSBG1 antibody 16077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Endocrinology

mTOR regulates mineralocorticoid receptor transcriptional activity by ULK1- dependent and independent mechanisms

Authors - Yusuf Ali
View Article