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  • Featured Product
  • KD/KO Validated

ACSF3 Polyclonal antibody

ACSF3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25484-1-AP

Print datasheet

Synonyms

PSEC0197, Malonate--CoA ligase ACSF3, mitochondrial, EC:6.2.1.76, Acyl-CoA synthetase family member 3

验证数据展示

  • WB analysis using 25484-1-AP

    WB analysis using 25484-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25484-1-AP (ACSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 25484-1-AP

    IHC staining of human stomach using 25484-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25484-1-AP (ACSF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25484-1-AP targets ACSF3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ACSF3 fusion protein Ag21979 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-CoA synthetase family member 3
Synonyms PSEC0197, Malonate--CoA ligase ACSF3, mitochondrial, EC:6.2.1.76, Acyl-CoA synthetase family member 3
Calculated Molecular Weight 576 aa, 64 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC028399
Gene Symbol ACSF3
Gene ID (NCBI) 197322
RRIDAB_2880099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ4G176
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACSF3 antibody 25484-1-APDownload protocol
IHC protocol for ACSF3 antibody 25484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bioconjug Chem

Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.

Authors - Yipeng Du
  • KD Validated
View Article
mouseWB

Sci Transl Med

Aberrant methylmalonylation underlies methylmalonic acidemia and is attenuated by an engineered sirtuin

Authors - PamelaSara E Head
View Article