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ACSL3 Polyclonal antibody
ACSL3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, LNCaP cells, HuH-7 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 14 publications below |
IHC | See 4 publications below |
产品信息
20710-1-AP targets ACSL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | acyl-CoA synthetase long-chain family member 3 |
Synonyms | EC:6.2.1.3, EC:6.2.1.2, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 3 |
Calculated Molecular Weight | 80 kDa |
Observed Molecular Weight | 70-80 kDa |
GenBank Accession Number | NM_004457 |
Gene Symbol | ACSL3 |
Gene ID (NCBI) | 2181 |
RRID | AB_10697670 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O95573 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ACSL3 antibody 20710-1-AP | Download protocol |
IHC protocol for ACSL3 antibody 20710-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Commun (Lond) Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma | ||
Clin Transl Med NAT10: An RNA cytidine transferase regulates fatty acid metabolism in cancer cells | ||
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. | ||
Biochim Biophys Acta Mol Cell Biol Lipids Rab18 binds PLIN2 and ACSL3 to mediate lipid droplet dynamics. | ||