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验证数据展示

  • WB analysis using 30214-1-AP

    WB analysis using 30214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30214-1-AP (ACSL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of LNCaP using 30214-1-AP

    IP experiment of LNCaP using 30214-1-AP

    IP result of anti-ACSL3 (IP:30214-1-AP, 4ug; Detection:30214-1-AP 1:4000) with LNCaP cells lysate 1360 ug.

ACSL3 Polyclonal antibody

ACSL3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 30214-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IP, ELISA

别名

ACS3, ACSL3, FACL3, LACS 3, LACS3, PRO2194

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, HuH-7 cells, LNCaP cells, MKN-45 cells, mouse kidney tissue
Positive IP detected inLNCaP cells
Planning an IHC experiment? We recommend our IHCeasy ACSL3 Ready-To-Use IHC Kit. ACSL3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30214-1-AP targets ACSL3 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 Human, mouse
免疫原 ACSL3 fusion protein Ag33005 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA synthetase long-chain family member 3
别名 ACS3, ACSL3, FACL3, LACS 3, LACS3, PRO2194
计算分子量 80 kDa
观测分子量72 kDa
GenBank蛋白编号BC041692
基因名称 ACSL3
Gene ID (NCBI) 2181
RRIDAB_2935532
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95573
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACSL3, also named as ACS3, FACL3 and LACS3, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL3 mediates hepatic lipogenesis. Preferentially uses myristate, laurate, arachidonate and eicosapentaenoate as substrates. Has mainly an anabolic role in energy metabolism. Required for the incorporation of fatty acids into phosphatidylcholine, the major phospholipid located on the surface of VLDL (very low density lipoproteins). The antibody is specific to ACSL3.

实验方案

Product Specific Protocols
WB protocol for ACSL3 antibody 30214-1-APDownload protocol
IP protocol for ACSL3 antibody 30214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols