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  • Featured Product
  • KD/KO Validated

ACSL4/FACL4 Recombinant antibody, PBS Only

ACSL4/FACL4 Uni-rAbTM Recombinant Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3I9

Cat no : 81196-1-PBS

Print datasheet

Synonyms

ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68

验证数据展示

  • WB analysis using 81196-1-RR (same clone as 81196-1-PBS)

    WB analysis using 81196-1-RR (same clone as 81196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81196-1-RR (ACSL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81196-1-RR (same clone as 81196-1-PBS)

    WB analysis of HepG2 using 81196-1-RR (same clone as 81196-1-PBS)

    WB result of ACSL4 antibody (81196-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IP experiment of HeLa using 81196-1-RR (same clone as 81196-1-PBS)

    IP experiment of HeLa using 81196-1-RR (same clone as 81196-1-PBS)

    IP result of anti-ACSL4/FACL4 (IP:81196-1-RR, 4ug; Detection:81196-1-RR 1:3000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
Planning an IHC experiment? We recommend our IHCeasy ACSL4 Ready-To-Use IHC Kit. ACSL4 primary antibody included.

产品信息

81196-1-PBS targets ACSL4/FACL4 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IP, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ACSL4/FACL4 fusion protein Ag18085 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acyl-CoA synthetase long-chain family member 4
Synonyms ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68
Calculated Molecular Weight 711 aa, 79 kDa
Observed Molecular Weight70 kDa, 75 kDa
GenBank Accession NumberBC034959
Gene Symbol ACSL4
Gene ID (NCBI) 2182
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60488
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).