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验证数据展示

  • WB analysis using 81196-1-RR (same clone as 81196-1-PBS)

    WB analysis using 81196-1-RR (same clone as 81196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81196-1-RR (ACSL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81196-1-RR (same clone as 81196-1-PBS)

    WB analysis of HepG2 using 81196-1-RR (same clone as 81196-1-PBS)

    WB result of ACSL4 antibody (81196-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACSL4 transfected HepG2 cells. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of human stomach cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of human liver cancer using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IP experiment of HeLa using 81196-1-RR (same clone as 81196-1-PBS)

    IP experiment of HeLa using 81196-1-RR (same clone as 81196-1-PBS)

    IP result of anti-ACSL4/FACL4 (IP:81196-1-RR, 4ug; Detection:81196-1-RR 1:3000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    IHC staining of rat brain using 81196-1-RR (same clone as 81196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81196-1-RR (ACSL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACSL4 antibody included
  • Featured Product
  • KD/KO验证

ACSL4/FACL4 Recombinant antibody, PBS Only

ACSL4/FACL4 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 81196-1-PBS

产品说明书

CloneNo. 3I9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

ACSL4, FACL4, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ACSL4 Ready-To-Use IHC Kit. ACSL4 primary antibody included.

产品信息

81196-1-PBS targets ACSL4/FACL4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACSL4/FACL4 fusion protein Ag18085 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase long-chain family member 4
别名 ACSL4, FACL4, EC:6.2.1.15, Arachidonate--CoA ligase, ACSL 4
计算分子量 711 aa, 79 kDa
观测分子量70 kDa, 75 kDa
GenBank蛋白编号BC034959
基因名称 ACSL4
Gene ID (NCBI) 2182
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60488
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acyl-CoA synthetase long-chain family member 4 (Acsl4), an important enzyme involved in lipid metabolism, participates in ferroptosis by converting free AA into arachidonoyl-CoA to generate lipid hydroperoxides. Recent studies revealed that ACSL4 is involved in biological responses including inflammation, steroidogenesis, cell death, female fertility, and cancer. Acsl4 has two isoforms: 79 kDa and 75 kDa. ACSL4 also can be detected in 70 kDa(PMID:35326233).