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ACSL5 Polyclonal antibody, PBS Only

ACSL5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15708-1-PBS

Print datasheet

Synonyms

LACS 5, FACL5, EC:6.2.1.3, EC:6.2.1.15, Arachidonate--CoA ligase

验证数据展示

  • WB analysis using 15708-1-AP (same clone as 15708-1-PBS)

    WB analysis using 15708-1-AP (same clone as 15708-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15708-1-AP (ACSL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 15708-1-AP (same clone as 15708-1-PBS)

    IP experiment of mouse brain using 15708-1-AP (same clone as 15708-1-PBS)

    IP result of anti-ACSL5 (IP:15708-1-AP, 4ug; Detection:15708-1-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15708-1-AP (ACSL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    IHC staining of human colon cancer using 15708-1-AP (same clone as 15708-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 15708-1-AP (ACSL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15708-1-PBS in a different storage buffer formulation.

产品信息

15708-1-PBS targets ACSL5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACSL5 fusion protein Ag8258 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-CoA synthetase long-chain family member 5
Synonyms LACS 5, FACL5, EC:6.2.1.3, EC:6.2.1.15, Arachidonate--CoA ligase
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC007985
Gene Symbol ACSL5
Gene ID (NCBI) 51703
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULC5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acyl-CoA synthetase 5, a member of the ACSL gene family that catalyzes the activation of long-chain fatty acids for lipid biosynthesis, is the only ACSL isoform that is both, located on mitochondria and functionally involved in enterocyte apoptosis.ACSL5 could play a role in promoting fatty acid-induced lipoapoptosis in hepatocytes as important mechanism in fatty liver-related disorders(PMID:20470896). ACSL5 is most abundant in liver, brown adipose tissue, and intestine and is located on both the mitochondrial membrane and endoplasmic reticulum(PMID:20798351). It has 3 isoforms produced by alternative splicing.