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ACSM5 Monoclonal antibody, PBS Only

ACSM5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5D6

Cat no : 67334-1-PBS

Print datasheet

Synonyms

ACSM5, FLJ20581, MACS3

验证数据展示

  • WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

    WB analysis of 4T1 using 67334-1-Ig (same clone as 67334-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 67334-1-Ig (same clone as 67334-1-PBS)

    WB analysis of rat liver using 67334-1-Ig (same clone as 67334-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67334-1-Ig (ACSM5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    IHC staining of human liver cancer using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67334-1-Ig (ACSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67334-1-Ig (same clone as 67334-1-PBS)

    IF Staining of HepG2 using 67334-1-Ig (same clone as 67334-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACSM5 antibody (67334-1-Ig, Clone: 2D5D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67334-1-PBS in a different storage buffer formulation.

产品信息

67334-1-PBS targets ACSM5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ACSM5 fusion protein Ag10056 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name acyl-CoA synthetase medium-chain family member 5
Synonyms ACSM5, FLJ20581, MACS3
Calculated Molecular Weight 208 aa, 23 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC016703
Gene Symbol ACSM5
Gene ID (NCBI) 54988
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6NUN0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.