• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ACSM5 Recombinant antibody

ACSM5 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241400E7

Cat no : 84444-1-RR

Print datasheet

Synonyms

MACS3, EC:6.2.1.2, Acyl-coenzyme A synthetase ACSM5, mitochondrial, Acyl-CoA synthetase medium-chain family member 5, ACSM 5

验证数据展示

  • WB analysis using 84444-1-RR

    WB analysis using 84444-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84444-1-RR (ACSM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 84444-1-RR

    FC experiment of HepG2 using 84444-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSM5 Recombinant antibody (84444-1-RR, Clone:241400E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84444-1-RR

    Affinity and Kinetic Characterization of 84444-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84444-1-RR against Human ACSM5 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue, human kidney tissue
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84444-1-RR targets ACSM5 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACSM5 fusion protein Ag9373 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acyl-CoA synthetase medium-chain family member 5
Synonyms MACS3, EC:6.2.1.2, Acyl-coenzyme A synthetase ACSM5, mitochondrial, Acyl-CoA synthetase medium-chain family member 5, ACSM 5
Calculated Molecular Weight 208 aa, 23 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC016703
Gene Symbol ACSM5
Gene ID (NCBI) 54988
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6NUN0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACSM5(Acyl-CoA synthetase medium-chain family member 5) is also named as MACS3 and belongs to the ATP-dependent AMP-binding enzyme family. ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACSM5 antibody 84444-1-RRDownload protocol
FC protocol for ACSM5 antibody 84444-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols