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ACSS1 Polyclonal antibody

ACSS1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17138-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IP, IHC, ELISA and More (1)

ACAS2L, ACECS1, AceCS2L, Acetyl coenzyme A synthetase 2 like, mitochondrial, ACSS1, KIAA1846

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCaco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17138-1-AP targets ACSS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 ACSS1 fusion protein Ag10896 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA synthetase short-chain family member 1
别名 ACAS2L, ACECS1, AceCS2L, Acetyl coenzyme A synthetase 2 like, mitochondrial, ACSS1, KIAA1846
计算分子量 689 aa, 75 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC039261
基因名称 ACSS1
Gene ID (NCBI) 84532
RRIDAB_2289182
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUB1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.

实验方案

Product Specific Protocols
WB protocol for ACSS1 antibody 17138-1-APDownload protocol
IHC protocol for ACSS1 antibody 17138-1-APDownload protocol
IP protocol for ACSS1 antibody 17138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
humanWB,IHC

Nat Commun

PMID: 27357947

Authors - Xue Gao
humanWB

EMBO J

Sex-specific, reciprocal regulation of ERα and miR-22 controls muscle lipid metabolism in male mice.

Authors - Judith Schweisgut
pigIF,WB

J Cell Physiol

Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.

Authors - Wenjun Zhou
  • KO Validated
humanWB, IHC

PLoS Comput Biol

Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples.

Authors - Xiaoqiang Sun
ratWB

Front Pharmacol

Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia.

Authors - Han-Qing Pang
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