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ACTA1-Specific Recombinant antibody, PBS Only

ACTA1-Specific Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, pig, chicken

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B15

Cat no : 82714-5-PBS

Print datasheet

Synonyms

ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle

验证数据展示

  • WB analysis using 82714-5-RR (same clone as 82714-5-PBS)

    WB analysis using 82714-5-RR (same clone as 82714-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82714-5-RR (ACTA1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

    IF Staining of mouse skeletal muscle using 82714-5-RR (same clone as 82714-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTA1-Specific antibody (82714-5-RR, Clone: 5B15 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82714-5-PBS in a different storage buffer formulation.

产品信息

82714-5-PBS targets ACTA1-Specific in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse, rat, pig, chicken samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity mouse, rat, pig, chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name actin, alpha 1, skeletal muscle
Synonyms ACTA1, Alpha Actin, α actin, Actin, alpha skeletal muscle, Actin alpha skeletal muscle
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 39-42 kDa
GenBank Accession NumberNM_001100
Gene Symbol ACTA1
Gene ID (NCBI) 58
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68133
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.