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ACTN3 Polyclonal antibody

ACTN3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 24378-1-AP

Print datasheet

Synonyms

actinin, alpha 3, ACTN3, Alpha actinin 3, F actin cross linking protein

验证数据展示

  • WB analysis of mouse skeletal muscle using 24378-1-AP

    WB analysis of mouse skeletal muscle using 24378-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24378-1-AP (ACTN3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 24378-1-AP

    IHC staining of mouse skeletal muscle using 24378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 24378-1-AP

    IHC staining of mouse skeletal muscle using 24378-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24378-1-AP (ACTN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 24378-1-AP

    IF Staining of mouse skeletal muscle using 24378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (24378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse skeletal muscle using 24378-1-AP

    IF Staining of mouse skeletal muscle using 24378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using ACTN3 antibody (24378-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24378-1-AP targets ACTN3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityrat
Immunogen ACTN3 fusion protein Ag19479 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actinin, alpha 3
Synonyms actinin, alpha 3, ACTN3, Alpha actinin 3, F actin cross linking protein
Calculated Molecular Weight 901 aa, 103 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC099647
Gene Symbol ACTN3
Gene ID (NCBI) 89
RRIDAB_2879515
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08043
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTN3 antibody 24378-1-APDownload protocol
IHC protocol for ACTN3 antibody 24378-1-APDownload protocol
IF protocol for ACTN3 antibody 24378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Dokl Biochem Biophys

Early Deсline in Rat Soleus Passive Tension with Hindlimb Unloading: Inactivation of Cross-bridges or Activation of Calpains?

Authors - I O Petrova
View Article