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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66628-1-Ig (same clone as 66628-1-PBS)

    WB analysis using 66628-1-Ig (same clone as 66628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66628-1-Ig (ACTN4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66628-1-Ig (same clone as 66628-1-PBS)

    IHC staining of human breast cancer using 66628-1-Ig (same clone as 66628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66628-1-Ig (ACTN4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66628-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ACTN4 Monoclonal antibody, PBS Only

ACTN4 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66628-1-PBS

Print datasheet

CloneNo. 1E12B8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66628-1-PBS targets ACTN4 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ACTN4 fusion protein Ag13549 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actinin, alpha 4
Synonyms ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 105 and 80 kDa
GenBank Accession NumberBC005033
Gene Symbol ACTN4
Gene ID (NCBI) 81
RRIDAB_2881988
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43707
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 105 and 80 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)