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ACTR1B Polyclonal antibody

ACTR1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11023-1-AP

Print datasheet

Synonyms

Beta-centractin, Beta centractin, ARP1B, ACTR1A, Actin-related protein 1B

验证数据展示

  • WB analysis using 11023-1-AP

    WB analysis using 11023-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11023-1-AP (ACTR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11023-1-AP

    IHC staining of human breast cancer using 11023-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11023-1-AP (ACTR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11023-1-AP

    IHC staining of human breast cancer using 11023-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11023-1-AP (ACTR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:300
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11023-1-AP targets ACTR1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACTR1B fusion protein Ag1467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Synonyms Beta-centractin, Beta centractin, ARP1B, ACTR1A, Actin-related protein 1B
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC010090
Gene Symbol ACTR1B
Gene ID (NCBI) 10120
RRIDAB_2877742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42025
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTR1B (also known as beta centractin) is a part of the 20S dynactin complex. It colocalizes with centrosomes and vesicular (membranous) structures and is involved in cell division. ACTR1B and ACTR1A share 90% amino acid identity, and this antibody recognizes both ACTR1B and ACTR1A.

实验方案

Product Specific Protocols
WB protocol for ACTR1B antibody 11023-1-APDownload protocol
IHC protocol for ACTR1B antibody 11023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols