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ACTR1B Polyclonal antibody

ACTR1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11023-1-AP

Print datasheet

Synonyms

Beta-centractin, Beta centractin, ARP1B, ACTR1A, Actin-related protein 1B

验证数据展示

  • WB analysis using 11023-1-AP

    WB analysis using 11023-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11023-1-AP (ACTR1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 11023-1-AP

    IHC staining of human breast cancer using 11023-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11023-1-AP (ACTR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 11023-1-AP

    IHC staining of human breast cancer using 11023-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11023-1-AP (ACTR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 11023-1-AP

    IF Staining of MCF-7 using 11023-1-AP

    Immunofluorescent analysis of MCF-7 cells, using ACTR1B antibody 11023-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:300
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11023-1-AP targets ACTR1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACTR1B fusion protein Ag1467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
Synonyms Beta-centractin, Beta centractin, ARP1B, ACTR1A, Actin-related protein 1B
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC010090
Gene Symbol ACTR1B
Gene ID (NCBI) 10120
RRIDAB_2877742
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42025
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTR1B (also known as beta centractin) is a part of the 20S dynactin complex. It colocalizes with centrosomes and vesicular (membranous) structures and is involved in cell division. ACTR1B and ACTR1A share 90% amino acid identity, and this antibody recognizes both ACTR1B and ACTR1A.

实验方案

Product Specific Protocols
WB protocol for ACTR1B antibody 11023-1-APDownload protocol
IHC protocol for ACTR1B antibody 11023-1-APDownload protocol
IF protocol for ACTR1B antibody 11023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols