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ACTR3B Polyclonal antibody

ACTR3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17022-1-AP

Print datasheet

Synonyms

Actin-related protein ARP4, Actin-related protein 3B, Actin-like protein 3B, Actin related protein ARP4, Actin related protein 3B

验证数据展示

  • WB analysis using 17022-1-AP

    WB analysis using 17022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17022-1-AP (ACTR3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 17022-1-AP

    WB analysis of A431 using 17022-1-AP

    WB result of ACTR3B antibody (17022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACTR3B transfected A431 cells.

  • IHC staining of human stomach cancer using 17022-1-AP

    IHC staining of human stomach cancer using 17022-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 17022-1-AP (ACTR3B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 17022-1-AP

    IHC staining of human stomach cancer using 17022-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 17022-1-AP (ACTR3B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, mouse pancreas tissue, HepG2 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17022-1-AP targets ACTR3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACTR3B fusion protein Ag10218 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ARP3 actin-related protein 3 homolog B (yeast)
Synonyms Actin-related protein ARP4, Actin-related protein 3B, Actin-like protein 3B, Actin related protein ARP4, Actin related protein 3B
Calculated Molecular Weight 418 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC008682
Gene Symbol ACTR3B
Gene ID (NCBI) 57180
RRIDAB_2305069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P1U1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTR3B antibody 17022-1-APDownload protocol
IHC protocol for ACTR3B antibody 17022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols