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验证数据展示

  • WB analysis using 17022-1-AP

    WB analysis using 17022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17022-1-AP (ACTR3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 17022-1-AP

    WB analysis of A431 using 17022-1-AP

    WB result of ACTR3B antibody (17022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACTR3B transfected A431 cells.

  • IHC staining of human stomach cancer using 17022-1-AP

    IHC staining of human stomach cancer using 17022-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 17022-1-AP (ACTR3B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 17022-1-AP

    IHC staining of human stomach cancer using 17022-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 17022-1-AP (ACTR3B antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ACTR3B Polyclonal antibody

ACTR3B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17022-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Actin-related protein ARP4, Actin-related protein 3B, Actin-like protein 3B, Actin related protein ARP4, Actin related protein 3B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, mouse pancreas tissue, HepG2 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17022-1-AP targets ACTR3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACTR3B fusion protein Ag10218 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ARP3 actin-related protein 3 homolog B (yeast)
别名 Actin-related protein ARP4, Actin-related protein 3B, Actin-like protein 3B, Actin related protein ARP4, Actin related protein 3B
计算分子量 418 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC008682
基因名称 ACTR3B
Gene ID (NCBI) 57180
RRIDAB_2305069
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P1U1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTR3B antibody 17022-1-APDownload protocol
IHC protocol for ACTR3B antibody 17022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols