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ACTR3B Monoclonal antibody

ACTR3B Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12C6

Cat no : 68037-1-Ig

Print datasheet

Synonyms

1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B, Actin-related protein 3B, Actin-related protein ARP4, ACTR3B, ARP11, ARP3 beta, ARP3BETA, ARP3-beta, ARP4

验证数据展示

  • WB analysis using 68037-1-Ig

    WB analysis using 68037-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68037-1-Ig

    WB analysis using 68037-1-Ig

    Brain tissues were subjected to SDS PAGE followed by western blot with 68037-1-Ig (ACTR3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse brain using 68037-1-Ig

    IHC staining of mouse brain using 68037-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68037-1-Ig

    IHC staining of mouse brain using 68037-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68037-1-Ig (ACTR3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMOLT-4 cells, pig brain tissue, Jurkat cells, K-562 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68037-1-Ig targets ACTR3B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen ACTR3B fusion protein Ag10218 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ARP3 actin-related protein 3 homolog B (yeast)
Synonyms 1G12C6, Actin like protein 3B, Actin related protein 3B, Actin related protein ARP4, Actin-like protein 3B, Actin-related protein 3B, Actin-related protein ARP4, ACTR3B, ARP11, ARP3 beta, ARP3BETA, ARP3-beta, ARP4
Calculated Molecular Weight 418 aa, 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC008682
Gene Symbol ACTR3B
Gene ID (NCBI) 57180
RRIDAB_2918779
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P1U1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTR3B antibody 68037-1-IgDownload protocol
IHC protocol for ACTR3B antibody 68037-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols