验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16992-1-AP targets ACTRT2 in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ACTRT2 fusion protein Ag10597 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin-related protein T2 |
| 别名 | actin related protein T2, Actin-related protein M2, Actin-related protein T2, ARPM2, ARP-T2 |
| 计算分子量 | 376 aa, 42 kDa |
| 观测分子量 | 40-42 kDa |
| GenBank蛋白编号 | BC029499 |
| 基因名称 | ACTRT2 |
| Gene ID (NCBI) | 140625 |
| RRID | AB_2878338 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TDY3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACTRT2 antibody 16992-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hum Reprod Deletion of ACTRT1 is associated with male infertility as sperm acrosomal ultrastructural defects and fertilization failure in human | ||
Development Loss of perinuclear theca ACTRT1 causes acrosome detachment and severe male subfertility in mice. | ||
Cell Rep The perinuclear theca protein Calicin helps shape the sperm head and maintain the nuclear structure in mice. |