ADA Polyclonal antibody
ADA Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, Sp2/0 cells |
| Positive IHC detected in | human stomach tissue, human cervical cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse small intestine tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| IHC | See 2 publications below |
产品信息
13328-1-AP targets ADA in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | ADA fusion protein Ag4128 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | adenosine deaminase |
| Synonyms | EC:3.5.4.4, ADA1 |
| Calculated Molecular Weight | 363 aa, 41 kDa |
| Observed Molecular Weight | 45-47 kDa |
| GenBank Accession Number | BC040226 |
| Gene Symbol | ADA |
| Gene ID (NCBI) | 100 |
| RRID | AB_2877939 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00813 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADA antibody 13328-1-AP | Download protocol |
| IHC protocol for ADA antibody 13328-1-AP | Download protocol |
| IF protocol for ADA antibody 13328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production. | ||
Eur J Pharmacol Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection. | ||
Cell Death Dis Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation | ||
bioRxiv The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma | ||
NPJ Biofilms Microbiomes Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model | ||
Animal Model Exp Med The cumulative analgesic effect of repeated electroacupuncture is modulated by Adora3 in the SCDH of mice with neuropathic pain |
