ADA Polyclonal antibody

ADA Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 13328-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

EC:3.5.4.4, ADA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Jurkat cells, Sp2/0 cells
Positive IHC detected inhuman stomach tissue, human cervical cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13328-1-AP targets ADA in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 ADA fusion protein Ag4128 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenosine deaminase
别名 EC:3.5.4.4, ADA1
计算分子量 363 aa, 41 kDa
观测分子量 45-47 kDa
GenBank蛋白编号BC040226
基因名称 ADA
Gene ID (NCBI) 100
RRIDAB_2877939
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenosine deaminase (ADA, EC 3.5.4.4), which has been found in plants, bacteria, and mammals including humans and others, catalyzes the hydrolysis of adenosine to yield inosine. It was demonstrated that ADA also catalyzes the hydrolysis of the derivatives of nucleoside adenosine such as 2′-deoxyadenosine. ADA is expressed on the surface of T and B cells and plays an important role in the development of immune system. (PMID: 26038697)

实验方案

Product Specific Protocols
WB protocol for ADA antibody 13328-1-APDownload protocol
IHC protocol for ADA antibody 13328-1-APDownload protocol
IF protocol for ADA antibody 13328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC

Front Cell Dev Biol

Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.

Authors - Xiaoting Chen
mouseWB

Eur J Pharmacol

Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.

Authors - Gerald Wölkart
mouseWB,IHC

Cell Death Dis

Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation

Authors - Mei-Xin Li
humanWB

bioRxiv

The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma

Authors - Ying-Jiun C Chen
WB

NPJ Biofilms Microbiomes

Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model

Authors - Yang Fu
mouseWB

Animal Model Exp Med

The cumulative analgesic effect of repeated electroacupuncture is modulated by Adora3 in the SCDH of mice with neuropathic pain

Authors - Faisal Ayub Kiani
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