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ADAL Polyclonal antibody

ADAL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27369-1-AP

Print datasheet

Synonyms

ADAL, adenosine deaminase like

验证数据展示

  • WB analysis using 27369-1-AP

    WB analysis using 27369-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27369-1-AP (ADAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 27369-1-AP

    IHC staining of human heart using 27369-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 27369-1-AP

    IHC staining of human heart using 27369-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, LNCaP cells, K-562 cells
Positive IHC detected inhuman heart tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27369-1-AP targets ADAL in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen ADAL fusion protein Ag25949 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenosine deaminase-like
Synonyms ADAL, adenosine deaminase like
Calculated Molecular Weight 267 aa, 30 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC075857
Gene Symbol ADAL
Gene ID (NCBI) 161823
RRIDAB_2880855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6DHV7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADAL antibody 27369-1-APDownload protocol
IHC protocol for ADAL antibody 27369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

EMBO J

Aberrant DNA N6 -methyladenine incorporation via adenylate kinase 1 is suppressed by ADAL deaminase-dependent 2'-deoxynucleotide pool sanitation

Authors - Shaokun Chen
View Article