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验证数据展示

  • WB analysis of THP-1 using 66620-1-Ig

    WB analysis of THP-1 using 66620-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66620-1-Ig (ADAM10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66620-1-Ig

    IHC staining of human prostate cancer using 66620-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66620-1-Ig (ADAM10 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ADAM10 antibody included

ADAM10 Monoclonal antibody

ADAM10 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66620-1-Ig

Print datasheet

CloneNo. 2A6B7
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Synonyms

AD10, ADAM 10, ADAM10, CD156c, CDw156, HsT18717, kuz, Kuzbanian protein homolog, MADM

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, MCF-7 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ADAM10 Ready-To-Use IHC Kit. ADAM10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66620-1-Ig targets ADAM10 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen ADAM10 fusion protein Ag23062 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADAM metallopeptidase domain 10
Synonyms AD10, ADAM 10, ADAM10, CD156c, CDw156, HsT18717, kuz, Kuzbanian protein homolog, MADM
Calculated Molecular Weight 748 aa, 84 kDa
Observed Molecular Weight 90-95 kDa
GenBank Accession NumberBC126253
Gene Symbol ADAM10
Gene ID (NCBI) 102
RRIDAB_2881980
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14672
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).

实验方案

Product Specific Protocols
WB protocol for ADAM10 antibody 66620-1-IgDownload protocol
IHC protocol for ADAM10 antibody 66620-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Arch Biochem Biophys

Maresin1 restrains chronic inflammation and Aβ production to ameliorate Alzheimer's disease via modulating ADAM10/17 and its associated neuroprotective signal pathways: A pilot study

Authors - Shuang Sun
  • KD Validated
View Article