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ADAM20 Polyclonal antibody

ADAM20 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20755-1-AP

Print datasheet

Synonyms

ADAM 20, ADAM20

验证数据展示

  • WB analysis using 20755-1-AP

    WB analysis using 20755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20755-1-AP (ADAM20 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20755-1-AP targets ADAM20 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ADAM20 fusion protein Ag14493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADAM metallopeptidase domain 20
Synonyms ADAM 20, ADAM20
Calculated Molecular Weight 776 aa, 87 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC025378
Gene Symbol ADAM20
Gene ID (NCBI) 8748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43506
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADAM20 is the functional equivalent of sperm fertilin-alpha (ADAM1), since that gene is nonfunctional in human. And northern blot analysis detected the 2.8-kb ADAM20 mRNA only in testis. The exclusive expression of ADAM20 in human testis and their sequence similarity with the fertilins suggest that it is expressed on sperm cells and involved in sperm maturation and/or fertilization(PMID:9469942). The full length protein has a singnal peptide, propeptide and 6 glycosylation sites.

实验方案

Product Specific Protocols
WB protocol for ADAM20 antibody 20755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols