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ADAM28 Polyclonal antibody
ADAM28 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse lung tissue, BxPC-3 cells, mouse colon tissue, mouse liver tissue, mouse small intestine tissue, mouse spleen tissue, PC-13 cells |
Positive IP detected in | mouse lung tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 6 publications below |
产品信息
The immunogen of 22234-1-AP is ADAM28 Fusion Protein expressed in E. coli.
Tested Applications | WB, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | ADAM28 fusion protein Ag17586 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ADAM metallopeptidase domain 28 |
Synonyms | ADAM 28, ADAM28, eMDC II, eMDCII, MDC L, MDC Lm, MDC Ls, MDCL |
Calculated Molecular Weight | 775 aa, 87 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC136478 |
Gene Symbol | ADAM28 |
Gene ID (NCBI) | 10863 |
RRID | AB_2879043 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UKQ2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ADAM28 antibody 22234-1-AP | Download protocol |
IP protocol for ADAM28 antibody 22234-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility | ||
Cancer Lett ADAM28 promotes tumor growth and dissemination of acute myeloid leukemia through IGFBP-3 degradation and IGF-I-induced cell proliferation.
| ||
Biol Reprod ADGRG2 is dispensable for lumicrine signalling regulating epididymal initial segment differentiation and gene expression† | ||
Mol Cell Neurosci Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR. | ||
J Reprod Dev Busulfan administration replicated the characteristics of the epididymal initial segment observed in mice lacking testis-epididymis lumicrine signaling |