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ADAM33 Polyclonal antibody

ADAM33 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22860-1-AP

Print datasheet

Synonyms

UNQ873/PRO1891, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 33, C20orf153, ADAM 33

验证数据展示

  • WB analysis using 22860-1-AP

    WB analysis using 22860-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22860-1-AP (ADAM33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, SK-BR-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22860-1-AP targets ADAM33 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ADAM33 fusion protein Ag18852 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADAM metallopeptidase domain 33
Synonyms UNQ873/PRO1891, EC:3.4.24.-, Disintegrin and metalloproteinase domain-containing protein 33, C20orf153, ADAM 33
Calculated Molecular Weight 813 aa, 88 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC125113
Gene Symbol ADAM33
Gene ID (NCBI) 80332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZ11
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADAM33 is a member of the 'a disintegrin and metalloprotease domain' family of proteins. Endogenous ADAM33 is detected in bronchus tissue, bronchial smooth muscle cells, and MRC-5 fibroblasts, consistent with a role in the pathophysiology of asthma(PMID:12535637). ADAM33 is a novel tumour suppressor gene that may be useful as a molecular marker for invasive lobular carcinoma of the breast(PMID:19267929)

实验方案

Product Specific Protocols
WB protocol for ADAM33 antibody 22860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols