验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy ADAMDEC1 Ready-To-Use IHC Kit. ADAMDEC1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17899-1-AP targets ADAMDEC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ADAMDEC1 fusion protein Ag12265 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADAM-like, decysin 1 |
| 别名 | EC:3.4.24.-, ADAM-like protein decysin-1, ADAMDEC 1, ADAM DEC1, A disintegrin and metalloproteinase domain-like protein decysin-1 |
| 计算分子量 | 470 aa, 53 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | BC074877 |
| 基因名称 | ADAMDEC1 |
| Gene ID (NCBI) | 27299 |
| RRID | AB_2878461 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15204 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Metalloendopeptidase ADAM-Like Decysin 1 (ADAMDEC1) was first identified as a unique member of the matrix metalloprotease (ADAM) family. ADAMs are increasingly recognized as playing an important role in gut homeostasis and bowel inflammation. ADAMDEC1 is abundantly, and almost exclusively, expressed in the gastrointestinal (GI) tract.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAMDEC1 antibody 17899-1-AP | Download protocol |
| IHC protocol for ADAMDEC1 antibody 17899-1-AP | Download protocol |
| IF protocol for ADAMDEC1 antibody 17899-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arch Toxicol A transcriptome-wide association study integrating multi-omics bioinformatics and Mendelian randomization reveals the prognostic value of ADAMDEC1 in colon cancer | ||
J Inflamm Res The m6A/m1A/m5C-Related Methylation Modification Patterns and Immune Landscapes in Rheumatoid Arthritis and Osteoarthritis Revealed by Microarray and Single-Cell Transcriptome | ||
Thorac Cancer Upregulation of ADAMDEC1 correlates with tumor progression and predicts poor prognosis in non-small cell lung cancer (NSCLC) via the PI3K/AKT pathway. | ||
Aging (Albany NY) An immune signature to predict the prognosis of ATRX-wildtype glioma patients and guide immune checkpoint blockade therapy | ||
Exp Cell Res ADAMDEC1 induces EMT and promotes colorectal cancer cells metastasis by enhancing Wnt/β-catenin signaling via negative modulation of GSK3β
| ||
Funct Integr Genomics Knockdown of ADAMDEC1 ameliorates ox-LDL-induced endothelial cell injury and atherosclerosis progression |
