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验证数据展示

  • WB analysis of MCF-7 using 82985-1-RR

    WB analysis of MCF-7 using 82985-1-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 82985-1-RR (ADAMDEC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82985-1-RR

    Affinity and Kinetic Characterization of 82985-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82985-1-RR against Human ADAMDEC1 were performed. The affinity constant is 19.4 pM.

ADAMDEC1 Recombinant antibody

ADAMDEC1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82985-1-RR
CloneNo. 230367F9

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

EC:3.4.24.-, ADAM-like protein decysin-1, ADAMDEC 1, ADAM DEC1, A disintegrin and metalloproteinase domain-like protein decysin-1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ADAMDEC1 Ready-To-Use IHC Kit. ADAMDEC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82985-1-RR targets ADAMDEC1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen ADAMDEC1 fusion protein Ag12265 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ADAM-like, decysin 1
Synonyms EC:3.4.24.-, ADAM-like protein decysin-1, ADAMDEC 1, ADAM DEC1, A disintegrin and metalloproteinase domain-like protein decysin-1
Calculated Molecular Weight 470 aa, 53 kDa
Observed Molecular Weight48-53 kDa
GenBank Accession NumberBC074877
Gene Symbol ADAMDEC1
Gene ID (NCBI) 27299
RRIDAB_3670735
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Metalloendopeptidase ADAM-Like Decysin 1 (ADAMDEC1) was first identified as a unique member of the matrix metalloprotease (ADAM) family. ADAMs are increasingly recognized as playing an important role in gut homeostasis and bowel inflammation. ADAMDEC1 is abundantly, and almost exclusively, expressed in the gastrointestinal (GI) tract.

实验方案

Product Specific Protocols
WB protocol for ADAMDEC1 antibody 82985-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols