验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, K-562 cells, HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31075-1-AP targets ADAMTS14 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ADAMTS14 fusion protein Ag34638 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADAM metallopeptidase with thrombospondin type 1 motif, 14 |
| 别名 | |
| 计算分子量 | 134 kDa |
| 观测分子量 | 134 kDa |
| GenBank蛋白编号 | NM_080722 |
| 基因名称 | ADAMTS14 |
| Gene ID (NCBI) | 140766 |
| RRID | AB_3669843 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8WXS8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation, and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS14 possesses high similarity with ADAMTS2 and ADAMTS3 in sequence and domain composition (PMID: 11741898). The expression of ADAMTS14 is correlated with the occurrence and progression of colorectal cancer and oral squamous cell carcinoma (PMID: 34856162; PMID: 32102222).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAMTS14 antibody 31075-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
