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ADAMTS14 Polyclonal antibody

ADAMTS14 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31075-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 31075-1-AP

    WB analysis using 31075-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31075-1-AP (ADAMTS14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells, HUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31075-1-AP targets ADAMTS14 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ADAMTS14 fusion protein Ag34638 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 14
Synonyms
Calculated Molecular Weight134 kDa
Observed Molecular Weight134 kDa
GenBank Accession NumberNM_080722
Gene Symbol ADAMTS14
Gene ID (NCBI) 140766
RRIDAB_3669843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8WXS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADAMTS (a disintegrin-like and metalloproteinase with thrombospondin motifs) is a family of extracellular metalloproteinases mediating diverse functions including matrix degradation, blood coagulation, and angiogenesis. The ADAMTS family constitutes a group of proteins composed of 19 enzymes and 7 ADAMTS-like proteins. ADAMTS14 possesses high similarity with ADAMTS2 and ADAMTS3 in sequence and domain composition (PMID: 11741898). The expression of ADAMTS14 is correlated with the occurrence and progression of colorectal cancer and oral squamous cell carcinoma (PMID: 34856162; PMID: 32102222).

实验方案

Product Specific Protocols
WB protocol for ADAMTS14 antibody 31075-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols