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验证数据展示

  • WB analysis using 31032-1-AP

    WB analysis using 31032-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31032-1-AP (ADAMTS2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 31032-1-AP

    IHC staining of mouse liver using 31032-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31032-1-AP (ADAMTS2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 31032-1-AP

    WB analysis using 31032-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31032-1-AP (ADAMTS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ADAMTS2 Polyclonal antibody

ADAMTS2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31032-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ADAMTS-2, pNPI, PCPNI, PCINP, ADAMTS 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A375 cells, PC-3 cells, THP-1 cell, COLO 205 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31032-1-AP targets ADAMTS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ADAMTS2 fusion protein Ag34957 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADAM metallopeptidase with thrombospondin type 1 motif, 2
别名 ADAMTS-2, pNPI, PCPNI, PCINP, ADAMTS 2
计算分子量135 kDa
观测分子量110~130 kDa
GenBank蛋白编号NM_014244
基因名称 ADAMTS2
Gene ID (NCBI) 9509
RRIDAB_3669823
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO95450
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

A disintegrin and metalloproteinase with thrombospondin motifs 2 (ADAMTS-2) is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. ADAMTS-2 can, like the procollagen C-proteinases, be regulated by transforming growth factor-β1 (TGF-β1), with implications for mechanisms whereby this growth factor effects net increases in formation of extracellular matrix. Mutations in the ADAMTS-2 gene cause dermatosparaxis in human (dermatosparactic type of Ehlers-Danlos syndrome, also known as Ehlers-Danlos syndrome type VIIC). Human ADAMTS-2 has eight potential sites for Asn-linked glycosylation and other mammals (PMID: 16046392, 12646579).

实验方案

Product Specific Protocols
WB protocol for ADAMTS2 antibody 31032-1-APDownload protocol
IHC protocol for ADAMTS2 antibody 31032-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols