验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human liver tissue, mouse lung tissue, mouse skeletal muscle tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human colon cancer tissue, human liver tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15304-1-AP targets ADAMTSL4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | ADAMTSL4 fusion protein Ag7781 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ADAMTS-like 4 |
| 别名 | ADAMTS like 4, ADAMTS like protein 4, ADAMTSL 4, ADAMTSL4, TSRC1, UNQ2803/PRO34012 |
| 计算分子量 | 117 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC027478 |
| 基因名称 | ADAMTSL4 |
| Gene ID (NCBI) | 54507 |
| RRID | AB_2222624 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UY14 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADAMTSL4 antibody 15304-1-AP | Download protocol |
| IHC protocol for ADAMTSL4 antibody 15304-1-AP | Download protocol |
| IP protocol for ADAMTSL4 antibody 15304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Matrix Biol Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families. | ||
Front Mol Biosci An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma. | ||
Oncotarget Correlation of five secretory proteins with the nasopharyngeal carcinoma metastasis and the clinical applications. | ||
Int J Nanomedicine Pioglitazone-Loaded Cartilage-Targeted Nanomicelles (Pio@C-HA-DOs) for Osteoarthritis Treatment |
