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验证数据展示

  • IHC staining of rat brain using 30153-1-AP

    IHC staining of rat brain using 30153-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30153-1-AP (ADCY5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30153-1-AP

    IHC staining of rat brain using 30153-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30153-1-AP (ADCY5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 30153-1-AP

    IF Staining of mouse brain using 30153-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ADCY5 antibody (30153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 30153-1-AP

    IF Staining of mouse brain using 30153-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ADCY5 antibody (30153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ADCY5 Polyclonal antibody

ADCY5 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 30153-1-AP

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Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA and More (1)

Synonyms

AC5, Adenylate cyclase type V, Adenylate cyclase type 5, adenylate cyclase 5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30153-1-AP targets ADCY5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ADCY5 fusion protein Ag32812 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenylate cyclase 5
Synonyms AC5, Adenylate cyclase type V, Adenylate cyclase type 5, adenylate cyclase 5
Calculated Molecular Weight139 kDa
GenBank Accession NumberNM_183357
Gene Symbol ADCY5
Gene ID (NCBI) 111
RRIDAB_3086248
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95622
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADCY5 belongs to the adenylate cyclase (ADCY) superfamily which is a group of glycoproteins regulating intracellular signaling (PMID: 35832555). ADCY5 is expressed only in neurons and all neurons express ADCY5 (PMID: 31970214). Gain-of-function mutations in ADCY5 is linked to a rare movement-onset disorder called ADCY5-related dyskinesia (PMID: 32627162).

实验方案

Product Specific Protocols
IHC protocol for ADCY5 antibody 30153-1-APDownload protocol
IF protocol for ADCY5 antibody 30153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Front Pharmacol

Comprehensively prognostic and immunological analyses of GLP-1 signaling-related genes in pan-cancer and validation in colorectal cancer

Authors - Chaojun Zhu
View Article